Outbreak of Salmonella serotype Hartford infections associated with unpasteurized orange juice.

Context: Acidic foods such as orange juice have been thought to be unlikely vehicles of foodborne illness.

Objective: To investigate an outbreak of Salmonella enterica serotype Hartford (Salmonella Hartford) infections among persons visiting a theme park in Orlando, Fla, in 1995.

Design: Review of surveillance data, matched case-control study, laboratory investigation, and environmental studies.

Comparison of a Rapid Plate Count and MPN Methods for Enumeration of Fecal Coliforms and Escherichia coli in Soft-Shell Clams.

A direct elevated temperature plate count method utilizing modified fecal coliform agar with rosolic acid (ETPC/mFC) was compared to 5-tube and 3-tube most probable number (MPN) procedures for its accuracy in enumerating fecal coliforms and Escherichia coli in naturally and artificially contaminated soft-shell clams ( Mya arenaria ). The results indicated that the extent of overall recovery of fecal coliforms was similar in the two methods tested. Therefore, the ETPC/mFC method may be considered as a rapid procedure for fecal coliform screening during depuration of soft-shell clams.

Enumeration of Vibrio parahaemolyticus and Vibrio vulnificus in Various Seafoods with Two Enrichment Broths.

This study compares recoveries of Vibrio parahaemolyticus and Vibrio vulnificus with salt-polymyxin B broth (SPB) and alkaline peptone water (APW) from samples of crab legs, oysters, shrimp, lobster and shark, which were inoculated at three levels (approximately 10 to 10, 10 to 10 and 10 to 10/g) with each of the pathogens. Six samples of each product were analyzed [3-tube most probable number (MPN)] with each broth. Inoculated samples of oysters and slurries of crab and lobster were also tested after cold stress (refrigerated at 2 to 4°C, 3 or 7 days, or frozen at -15°C for 21 or 28 days).

Comparison of Five Selective Enrichment Broths and Two Selective Agars For Recovery of Vibrio vulnificus From Oysters.

The Bacteriological Analytical Manual (6th edition) specifies use of glucose-salt-teepol (GST) broth for detection of Vibrio vulnificus and other halophilic vibrios in seafood. Since teepol is no longer commercially available, this study compared five enrichment broths for their ability to recover V. vulnificus .

Lipid globule staining to aid in differentiating Bacillus species.

The use of the lipid globule stain to aid in differentiating the Bacillus cereus group (i.e., B.