Identification of cellular signatures associated with chinese hamster ovary cell adaptation for secretion of antibodies.

The secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental bottleneck in the manufacturing of protein-based therapeutics. Unconventional biological drugs with complex structures and processing requirements are particularly problematic. Although engineered vector DNA elements can achieve rapid and high-level therapeutic protein production, a high metabolic and protein folding burden is imposed on the host cell.

A Recyclable Polypropylene Multilayer Film Maintaining the Quality and the Aroma of Coffee Pods during Their Shelf Life.

Films for coffee-pod packaging usually contain aluminium as an impermeable foil that is not recyclable and has to be discharged as waste. In this study, a recyclable polypropylene multilayer film is proposed as an alternative. The performance on the chemical composition of coffee was evaluated and compared to that of film containing aluminium (standard).

The Lipidic and Volatile Components of Coffee Pods and Capsules Packaged in an Alternative Multilayer Film.

Coffee pods and capsules require packaging that guarantees the optimal coffee preservation. The chemical composition of coffee can undergo quality decay phenomena during storage, especially in terms of lipidic and volatile components. Amongst coffee packaging, aluminum multilayer materials are particularly widely diffused.

Constraints on Tsallis Cosmology from Big Bang Nucleosynthesis and the Relic Abundance of Cold Dark Matter Particles.

By employing Tsallis' extensive but non-additive δ-entropy, we formulate the first two laws of thermodynamics for gravitating systems. By invoking Carathéodory's principle, we pay particular attention to the integrating factor for the heat one-form. We show that the latter factorizes into the product of thermal and entropic parts, where the entropic part cannot be reduced to a constant, as is the case in conventional thermodynamics, due to the non-additive nature of Sδ.