Rapid, automated assay for progesterone on the Abbott AxSYM analyzer.

We describe an automated assay for progesterone (P4) in human serum and plasma with the Abbott AxSYM random-access immunoassay analyzer. In this one-step competitive assay, P4 immobilized onto latex microparticles competes with sample P4 for binding to a conjugate of alkaline phosphatase (AP) and anti-P4 antibody. Total CVs ranged from 3.

Neuroendocrine carcinomas of the stomach: a clinicopathologic evaluation.

Background: The purpose of this study was to determine by immunocytochemistry the relative incidence and clinicopathologic characteristics of neuroendocrine carcinomas of the stomach.

Methods: Sections from paraffin blocks from 81 patients who had undergone resection of carcinomas of the stomach were immunostained with a battery of neuroendocrine differentiation markers and with A-80, a marker of exocrine differentiation. The clinical and pathologic data of the 12 patients diagnosed with neuroendocrine carcinomas of the stomach were analyzed.

Elevated plasma platelet-derived growth factor B-chain levels in cancer patients.

Platelet-derived growth factor (PDGF) is produced by a variety of normal and tumor cells in vitro. We have developed an enzyme-linked immunosorbent assay for the detection of the B-chain of PDGF. This assay can reliably detect 0.

Measurement of murine IgG and IgM concentrations in the SCLC MAb panel; effects of concentration on sensitivity and specificity.

Sensitive, solid-phase enzyme immunoassays for detecting murine IgM and IgG were developed and used to quantitate immunoglobulin concentrations in the SCLC MAb panel. Among the IgM MAbs, most contained between 10-75 micrograms ml-1. At least three IgM MAbs, however, contained less than 2 micrograms ml-1 and six contained greater than 75 micrograms ml-1.