Investigation of an LC-MS-MS (QTrap) method for the rapid screening and identification of drugs in postmortem toxicology whole blood samples.

The QTrap liquid chromatography-tandem mass spectrometry (LC-MS-MS) by Applied Biosystems was investigated as an adjund to enzyme immunoassay (EIA) for the rapid detection of drugs in blood. Thus, a procedure used to identify drugs in whole blood by EIA was extended to LC-MS-MS analysis. A multiple reaction monitoring (MRM) database of over 100 drugs was constructed to analyze for those drugs commonly observed in postmortem toxicology cases.

Shoulder disorders and postural stress in automobile assembly work.

Objectives: A case-referent study was conducted in an automobile assembly plant to evaluate the risk of shoulder disorders associated with nonneutral postures.

Methods: The cases were workers who reported shoulder pain to the plant clinic during a 10-month period and met symptom criteria (pain frequency or duration in the past year) in an interview; more than one-half also had positive findings in a physical examination. The referents were randomly selected workers who were free of shoulder disorders according to the clinic records, the interview, and the physical examination.

The frequency of heteroplasmy in the HVII region of mtDNA differs across tissue types and increases with age.

An immobilized sequence-specific oligonucleotide (SSO) probe system consisting of 16 SSO probes that detect sequence polymorphisms within five regions of the mtDNA control region was used to investigate the frequency of heteroplasmy in human mtDNA. Five regions of hypervariable region II (HVII) of the control region were studied in blood-, muscle-, heart-, and brain-tissue samples collected from 43 individuals during autopsy. An initial search for heteroplasmy was conducted by use of the SSO probe system.

Development and evaluation of an observational method for assessing repetition in hand tasks.

Several physical stressors, including repetitive, sustained, and forceful exertions, awkward postures, localized mechanical stress, highly dynamic movements, exposures to low temperatures, and vibration have been linked to increased risk of work-related musculoskeletal disorders. Repetitive exertions have been among the most widely studied of these stressors, but there is no single metric for assessing exposure to repetitive work. A new methodology enables repetitive hand activity to be rated based on observable characteristics of manual work.

Interlaboratory comparison of autoradiographic DNA profiling measurements. 3. Repeatability and reproducibility of restriction fragment length polymorphism band sizing, particularly bands of molecular size > 10K base pairs.

The observed interlaboratory standard deviation (SD) associated with the restriction fragment length polymorphism (RFLP) measurement of DNA fragment size is a predictable function of the observed mean band size (MBS). For DNA fragments of size 1,000 base pairs (bp) to the largest resolved component of commonly used "sizing ladder" calibration materials (about 20,000 bp), the variation in the sizing data from the Technical Working Group on DNA Analysis Methods (TWGDAM)-sponsored "Large Fragment Study" is well-described by SD = 7.5 (1 + MBS/19 500)7.