Simplified quantitative determination of phospholipids in amniotic fluid, alveolar lavages and milk using high performance liquid chromatography (HPLC).

A one step HPLC method for the determination of the main phospholipids, phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingomyelin (SP) and lysolecithin (LL) in amniotic fluid, milk, tracheal secretion, lung lavages, gastric aspirates, infant formulas and some other human and animal media has been developed. Quantitation occurs automatically using computer calculation and lysolecithin as an internal standard. Detection limits are at about 0.

Determination of the phospholipid content of human milk, cow's milk and various infant formulas.

The phospholipid (PL) content of human milk, cow's milk, and various infant formulas was determined by recently developed high performance liquid chromatography (6). As the examinations promised, the content of phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylcholine (PC), and sphingomyelin (SP) was not changed by homogenization and pasteurization of cow's milk. Levels of phosphatidylglycerol (PG) were below the detection limit.

Effect of blood and meconium on the determination of phospholipids in amniotic fluid using high pressure liquid chromatography.

This paper presents the evaluation of the effects of blood and meconium on the determination of the phospholipids phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingomyelin (SP) and lysolecithin (LL) in amniotic fluid. Phospholipids were analyzed by a new method using high pressure liquid chromatography (HPLC), which is based on the procedure of BRIAND et al. The method was extended for quantitative determination with lysolecithin as internal standard.

Effect of long-term treatment with antiepileptic drugs on the vitamin status.

The status of vitamins A, B1, B2, B6, B12, C, D, and E as well as that of beta-carotene, biotin, and folate in the blood of over 500 epileptics was compared with that of a normal population. Male and female epileptics showed a poorer supply of vitamins B2, biotin, folate, and 25-hydroxycholecalciferol; the males, of only vitamin B6, B12, and E, and the women, of only vitamin A. Concentrations of beta-carotene and vitamin E in female epileptics were higher.