Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography.

The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[35S] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared.

Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. II. Comments on the methods and type classification tests of E. rhusiopathiae strains.

The tube precipitation method with antigen produced from autoclaved organisms and those produced by CH3COOH extraction and with good antisera have proved to give the best results and should be preferred at least in the routine work. It is a quick method but the results cannot be fixed and later used in documentation. - The paper chromatographic method is very sensitive, makes much work and heavy demands on the clarity of the antigen and serum and reading the test.

Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. I. Methods and influence of some factors on their results.

To contribute to the standardization of typing Erysipelothrix rhusiopathiae strains the following points have been examined and compared: the usefulness of tube precipitation, paper chromatography and agar gel diffusion; different methods to produce good antisera (specific high titres) and antigens (CH3COOH-, HCl-extract, extraction by means of autoclavation). Rabbits have a considerably varying individual ability to produce Erysipelas antibodies with good precipitation titres. The antigenic effect of different E.

[Studies of the occurrence of erysipelas bacteria in healthy swine in the Hungarian People's Republic with reference to possible use of erysipelas live vaccines].

The number of pigs for slaughter which carried erysipelas bacteria has gone down continuously in Hungary. The incidence of erysipelas declined in parallel. That improving trend has been attributed to better conditions of large-stock keeping and to the exclusive use of inactivated vaccine.