Effects of oxygen on the antigenic landscape of prostate cancer cells.

Background: Use of allogeneic cancer cells-based immunotherapy for treatment of established prostate cancer (PCa) has only been marginally effective. One reason for failure could stem from the mismatch of antigenic signatures of vaccine cells and cancer in situ. Hence, it is possible that vaccine cells expressed antigens differently than tumor cells in situ.

Identification and characterization of HIV-1 latent viral reservoirs in peripheral blood.

Plasma viral load and CD4 counts are effective for clinical monitoring, but they do not give a full representation of HIV-1 quasispecies in cellular reservoirs, the major repository of replication-competent HIV-1 in infected individuals. We sought to develop a diagnostic system that might stimulate the replication-competent HIV-1 reservoirs for enhanced clinical monitoring, including selection of antiretroviral regimens. Whole-blood samples from 45 HIV-infected individuals were collected into 1 ViraStim HIV-1 activation tube and 1 EDTA tube.

Prognostic value of discs large homolog 7 transcript levels in prostate cancer.

Hypoxia has been associated with malignant progression, metastasis and resistance to therapy. Hence, we studied expression of hypoxia-regulated genes in 100 prostate cancer (CaP) bulk tissues and 71 adjacent benign tissues. We found 24 transcripts significantly overexpressed (p ≤ 0.

Nitrous oxide as a tracer gas in the ASHRAE 110-1995 Standard.

ANSI/ASHRAE Standard 110 provides a quantitative method for testing the performance of laboratory fume hoods. Through release of a known quantity (4.0 Lpm) of a tracer gas, and subsequent monitoring of the tracer gas concentration in the "breathing zone" of a mannequin positioned in front of the hood, this method allows for evaluation of laboratory hood performance.

Age-dependent response of murine female bone marrow cells to hyperbaric oxygen.

Consequences of age on the effects of hyperbaric oxygen (HBO) on bone marrow (BM) derived stem cells and progenitors (SCPs) are largely unknown. We treated 2- and 18-month old C57BL/6 female mice by HBO. Hematopoietic stem cells and progenitors, enumerated as colony-forming units in culture, were doubled only in peripheral leukocytes and BM cells of young mice receiving HBO.