[Effect of the structure of the nucleoside ligand of cobalamines on their enzymatic properties in a glycerol dehydratase system].

The interaction between 8 adenosyl cobalamine (AdoCbl) analogs modified in adenine and deoxyribose of the nucleoside ligand and glycerol dehydratase from Klebsiella pneumoniae ATCC 25955 was studied. It was found that araadenosyl-, 3-isoadenosyl-, aristeromycyl- and nebularyl cobalamines possess coenzymic properties. The catalytic activities of these analogs complexes with glycerol dehydratase make up to 110, 36, 30 and 6% of the enzyme activity with the natural cofactor AdoCbl.