Pharmacokinetic study of niosome encapsulated insulin.

Pharmacokinetic profile and hypoglycemic effect, after intraperitoneal injection of insulin and insulin encapsulated in niosomes were determined in diabetic rats. Niosomes (non-ionic surfactant vesicles) of different doses and different lipid compositions were prepared by lipid layer hydration method. Plasma samples were collected at specified time intervals and plasma concentration of insulin was determined by HPLC.

Rapid and sensitive method for determination of nimesulide in human plasma by high-performance liquid chromatography.

A rapid and sensitive high-performance liquid chromatographic method for determination of nimesulide in human plasma has been developed. The chromatographic system uses a reversed-phase C18 column with UV-Vis detection at 230 nm. Mobile-phase consisted of phosphate buffer (pH 5.

High-performance liquid chromatographic determination of insulin in rat and human plasma.

A rapid and simple isocratic chromatographic procedure for the determination of insulin in human and rat plasma using reversed-phase (RP) high-performance liquid chromatography with an ultraviolet/visible detector is described. The method includes extraction of insulin from human and rat plasma into dichloromethane, followed by back-extraction into 0.05 N hydrochloric acid.

Anti-inflammatory and anti-nociceptive activity of disodium glycyrrhetinic acid hemiphthalate.

The anti-inflammatory and anti-nociceptive effects of disodium glycyrrhetinic acid hemiphthalate (glycyrrhetinic acid 3 beta-O-hemiphthalate sodium) after parenteral administration were determined in several animal models. Disodium glycyrrhetinic acid hemiphthalate (GAP) significantly inhibited the rat paw edema induced by carrageenin (ID50 = 21.37 mg/kg), and mice writhing responses caused by acetic acid (ID50 = 9.