Publications by authors named "G Kh Umbetova"

Position-effect variegation in Drosophila melanogaster is accompanied by compaction of the corresponding chromosomal regions. The compaction can be continuous, so that bands and interbands located distal to the eu-heterochromatic junction fuse into one dense block, or discontinuous, when two or more zones of compaction are separated by morphologically and functionally normal regions. In this work it was found that in both continuous and discontinuous compaction the blocks of dense material contain the immunochemically detectable protein HP1, which has previously been characterized as specific for heterochromatin.

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A chromosomal region subjected to position effect variegation was analysed for possible DNA under-replication. DNA clones from the vicinity of the euheterochromatin junction and from a distance of hundreds of kilobase pairs were used as probes. Formation of compact blocks of chromatin is regarded as a characteristic feature of position effect variegation.

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With the help of in situ hybridization respective location of 33 microclones from the genomic library of the Drosophila melanogaster large band 10A1-2, 4 clones obtained by chromosomal walking and 18 chromosomal rearrangements with breakpoints in the limits of this band was determined. A DNA fragment homologous to poly(A)+ RNA from the 3rd instar larvae has been revealed. Summarizing the obtained and published data, at least 3 genes and 6 transcriptionally active fragments appear to be located in the 10A1-2 band.

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