Rapid induction of preneoplastic liver foci in embryonal turkey liver by diethylnitrosamine.

Diethylnitrosamine (DEN) was injected into fertilized turkey eggs. After incubation for 24 days, the livers of the embryos were removed and investigated by means of histological and enzyme-histochemical methods. Doses of 2 and 5 mg DEN per egg had no effect on mortality and embryo weight but induced various types of foci of altered hepatocytes.

Foci of altered hepatocytes induced in embryonal turkey liver.

Fertilized turkey eggs were injected with N-nitrosomopholine (1-10 mg/egg) or urethane (0.1-10 mg/egg) prior to or during the first hours of incubation. Four days prior to hatching, the experiments were terminated and the livers of the embryos removed.