[Adenovirus KR95, isolated from chickens during an outbreak of hydropericarditis, is the pathogen of this disease].

Virus agent KR95 was isolated from the liver of dieoff chickens during an outbreak of hydropericarditis syndrome at a poultry farm in Russia. Electron microscopic examination of the virus morphology, comparative restriction cleavage map construction, DNA-DNA hybridization, and analysis of structural proteins from purified and disrupted virions showed that the agent is to be classified as type 1 avian adenovirus.

[Analysis of core virion polypeptides from the pathogen causing chicken egg-drop syndrome].

The cores of egg-drop syndrome virus (EDS-76) were isolated by the pyridine technique. EDS-76 proved to be much more resistant to pyridine disruption than other adenoviruses and treatment with 10% pyridine did not lead to complete dissociation of capsid and cores; only increase of pyridine concentration to 20% produced satisfactory results. At least three polypeptides (24, 10.

[Expression of a gene encoding the porcine rotavirus VP7 capsid using a nondefective recombinant adenoviral genome].

A nondefective recombinant human adenovirus 5 (Ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein VP7 in the E3 region of the Ad5 genome has been obtained. mRNA for VP7 in recombinant Ad5KV14/VP7 infected cells was demonstrated by means of RT-PCR. Radioimmunoprecipitation of cell extracts infected with Ad5KV14/VP7 on early (12 h p.

[The intermolecular disulfide bonds of the surface polypeptides in the infectious laryngotracheitis virus of hens].

The envelope proteins of chicken infectious laryngotracheitis virus were investigated using immunoblotting, radioimmuno-precipitation, and diagonal SDS-PAGE. Polypeptides with molecular weights of 205, 155, 115, 90, and 60 kDa were detected by rabbit antiserum and those of 205, 115, 90, and 60 kDa by chicken antiserum. When chicken antiserum was used, the 60 kDa polypeptide appeared to be the major immunogen.