Immunohistochemical detection of macrophage migration inhibitory factor in fetal and adult bovine epididymis: release by the apocrine secretion mode?

Originally defined as a lymphokine inhibiting the random migration of macrophages, the macrophage migration inhibitory factor (MIF) is an important mediator of the host response to infection. Beyond its function as a classical cytokine, MIF is currently portrayed as a multifunctional protein with growth-regulating properties present in organ systems beyond immune cells. In previous studies, we detected substantial amounts of MIF in the rat epididymis and epididymal spermatozoa, where it appears to play a role during post-testicular sperm maturation and the acquisition of fertilization ability.

Mitochondrial malic enzyme: purification from bovine brain, generation of an antiserum, and immunocytochemical localization in neurons of rat brain.

To elucidate the cellular location of mitochondrial malic enzyme in brain, immunocytochemical studies were performed. For this purpose, mitochondrial malic enzyme was purified to apparent homogeneity from bovine brain and used for the immunization of rabbits. Subjecting the antiserum to affinity purification on immobilized antigen as an absorbent yielded a purified immunoreactive antibody preparation, which was characterized by probing cytosolic and mitochondrial fractions of bovine and rat brain in western blotting.

[Histologic reactions following implantation of a "lyodura-soft" band--animal experiment studies of an abdomino-vaginal sling operation].

Abdomino-vaginal sling operations are favoured for the treatment of female stress incontinence recidive. An actual material for the sling ribbon is the Lyodura-soft. Histological reactions between one week and one year are described after experimental subcutaneous implantation of the Lyodura-soft ribbon in rats.

[Light microscopic investigation of the tissue integration of Lyodura soft implants].

Commercial preparations of human "Lyodura soft" were implanted into the subcutaneous connective tissue of 40 Wistar rats. After survival times from 7 days up to 1 year the samples were examined by light microscopy. An initial reaction of mononuclear cells, activated fibroblasts, and some giant cells of foreign body type in the surrounding connective tissue is followed by the development of a fibrous capsule rarefied by cells around the implant in the time up to 3 months.