Automated production of high density cosmid and YAC colony filters using a robotic workstation.

We report here on a system for automated preparation of high-density colony filters of arrayed libraries using the high density replicating system (HDR) for the Beckman Biomek 1000 robotic workstation. This system, consisting of a 96-pin tool, a sterilization station and controlling software, transfers samples from microplates onto target membranes in arrays up to 36 times the density of a 96-well microplate. The transfer operation can be completely automated with the addition of the Biomek Side Loader System, which consists of a robotic arm capable of transferring plates and filters between the Biomek working tablet and a storage area.

A rapid and preparative method for the separation of yeast ribosomal proteins by using high-performance liquid chromatography.

Ribosomal proteins from the yeast Saccharomyces cerevisiae were separated, on a preparative scale, by ion-exchange h.p.l.

Application of high-performance liquid chromatography to the purification and characterization of ribosomal protein L3 from trichodermin-resistant yeast mutants.

A new h.p.l.

Changes in histone acetylation during the development of rabbit bone marrow erythroid cells.

The acetylation of histones in rabbit bone marrow erythroid cells was investigated by measuring the incorporation of labelled acetate into erythroblasts which were separated by velocity sedimentation at unit gravity into five fractions corresponding to different stages of development. Histone acetylation decreased during erythroid development in concert with a decline in DNA and histone synthesis. Some acetylation persisted after condensation of the nucleus and cessation of DNA synthesis in late orthochromatic cells.