Light-driven post-translational installation of reactive protein side chains.

Post-translational modifications (PTMs) greatly expand the structures and functions of proteins in nature. Although synthetic protein functionalization strategies allow mimicry of PTMs, as well as formation of unnatural protein variants with diverse potential functions, including drug carrying, tracking, imaging and partner crosslinking, the range of functional groups that can be introduced remains limited. Here we describe the visible-light-driven installation of side chains at dehydroalanine residues in proteins through the formation of carbon-centred radicals that allow C-C bond formation in water.

Effect of microinterventional endocapsular nucleus disassembly using centripetal loop fragmentation on refractive outcomes after cataract surgery.

Purpose: To evaluate the refractive impact of centripetal fragmentation using the miLOOP system for nucleus disassembly, which minimizes lens zonulocapsular instability associated with endocapsular lens manipulation.

Setting: Private practice, Batesville, Indiana, USA.

Design: Retrospective comparative consecutive series.

Mediastinal cryptococcosis simulating thyroid neoplasia in immunocompetent patient with prior diagnosis and treatment for COPD.

We report a case of mediastinal cryptococcosis with thyroid invasion in a patient who presented dry cough and persistent dyspnea. This patient has been treated for COPD, presenting no clinical responses. Spirometry showed fixed flattening of inspiratory and expiratory loops, suggesting extrinsic tracheal compression.

High-Resolution Infrared Thermography of Esophageal Temperature During Radiofrequency Ablation of Atrial Fibrillation.

Background: Catheter ablation for atrial fibrillation has potential to cause esophageal thermal injury. Esophageal temperature monitoring during ablation is commonly used; however, it has not eliminated thermal injuries, possibly because conventional sensors have poor spatial sampling and response characteristics. To enhance understanding of temperature dynamics that may underlie esophageal injury, we tested a high-resolution, intrabody, infrared thermography catheter to continuously image esophageal temperatures during ablation.