GC-MS analysis of the leaf surface components of four Bulgarian grapevines grown under different conditions.

Four grapevine seedlings were propagated and planted both in a greenhouse and in an experimental field. Surface components of their fresh leaves were easily obtained and analysed by gas chromatography-mass spectrometry. In total, 21 surface metabolites were identified.

Surface metabolic composition of berries collected from 13 Bulgarian grapevine plants.

The surface metabolic composition of berries, collected from the Bulgarian grapevine variety Storgozia, and 12 of its seedlings were studied by GC/MS. Diverse surface metabolites were identified. The main components were long chain n-alkanes, terpenoids, and esterified long-chain fatty acids.

Seasonal variations in the chemical composition of vine-grape leaf surface.

Surface leaf metabolites of two seedlings of a Bulgarian winemaking cultivar Storgozia I(1) were analyzed in two seasons - summer (July) and autumn (October). The resistance towards some fungal pathogens of one of the plants was estimated as superior to the resistance of the other one. Significant seasonal variations in the chemical constituents of the two seedlings were observed.

Preliminary study on biomarkers for the fungal resistance in Vitis vinifera leaves.

We examined the leaf chemical composition of six seedlings obtained by self-pollination of the Bulgarian wine-making variety Storgozia as well as the cultivar Bouquet, which is the susceptible parent of Storgozia. The chemical composition was investigated in the framework of a program for identification of metabolites associated with disease resistance in grape-vine. Acetone, dichloromethane and butanol extracts, as well as volatiles obtained from fresh material were analyzed by GC/MS.