Incubation in vitro of rat liver nuclei in the presence of S-adenosyl[methyl-(3)H]methionine ([(3)H] SAM) leads to incorporation of the radioactive label not only into core-histones H3 and H4, but also into linker histone H1. Addition of distamycine A to the incubation medium stimulates label incorporation into histone H1 ~ in 6 times and into histone H3 ~ in 2 times. The presence of distamycine facilitates histone H1 extraction by polyglutamic acid (poly(Glu)) and decreases of UV-induced DNA-histone cross-links formation.
View Article and Find Full Text PDFThe dynamics of changes in total proteolytic activity and activities of various groups of proteases in the coleoptiles of 3- to 12-day-old wheat seedlings grown in light with and without antioxidant BHT (2,6-di-tert-butyl-4-methylphenol) was studied. It was established that the specialized proteases that easily hydrolyze specific synthetic substrates and the enzymes actively hydrolyzing histone H1 dominate in young coleoptiles of 3- to 4-day-old seedlings. Proteases that degrade equally well the majority of the studied substrates are accumulated in the cells of old coleoptiles of 11- to 12-day-old seedlings.
View Article and Find Full Text PDFWith vaccination having become usual practice in health care, the incidence rate of measles in the Donetsk region has fallen 53.6-fold versus the prevaccination period. Bet there are also negative processes in the epidemiology of measles; of these, ever more frequent occurrence of the infection under consideration in persons of mature years is worthy of particular mention.
View Article and Find Full Text PDFRecordable in Donets'k Province since the end of 1995 is an epidemic rise in incidence rates of HIV infection, with 3 to 3.5-fold annual increase in the number of children born to HIV-infected mothers. In 96.
View Article and Find Full Text PDFDNA-protein cross-links in white blood cells of Apodemus agrarius and Clethrionomys glareolus dwelling in the territory of the guarded zone of the radioactive waste storage in Sergievo Posad (area with gamma-background 50-400 microR/h) have been determined. As a control the animals from sanitary protected area of Sergievo Posadsky department (gamma-background on the soil surface is 8-10 microR/h) was used. It was found that the doses received by Apodemus agrarius and Clethrionomys glareolus, resulted in increasing of the DNA-protein cross-link level in the Apodemus agrarius white blood cells by a factor of 1.
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