[Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences].

Photochemical reactions in cell DNA are induced in various organisms by solar UV radiation and may lead to a series of biological responses to DNA damage, including apoptosis, mutagenesis, and carcinogenesis. The chemical nature and the amount of DNA lesions depend on the wavelength of UV radiation. UV type B (UVB, 290-320 nm) causes two main lesions, cyclobutane pyrimidine dimers (CPDs) and, with a lower yield, pyrimidine (6-4) pyrimidone photoproducts (6-4PPs).

Molecular Bases of Signaling Processes Regulated by Cryptochrome Sensory Photoreceptors in Plants.

The blue-light sensors, cryptochromes, compose the extensive class of flavoprotein photoreceptors, regulating signaling processes in plants underlying their development, growth, and metabolism. In several algae, cryptochromes may act not only as sensory photoreceptors but also as photolyases, catalyzing repair of the UV-induced DNA lesions. Cryptochromes bind FAD as the chromophore at the photolyase homologous region (PHR) domain and contain the cryptochrome C-terminal extension (CCE), which is absent in photolyases.

Signaling Mechanisms Regulating Diverse Plant Cell Responses to UVB Radiation.

UVB radiation (290-320 nm) causes diverse effects in plant cells that vary with the fluence rate of exposure. High fluence rates of UVB radiation cause damage to DNA and formation of reactive oxygen species in mitochondria and chloroplasts, which lead to oxidation of membrane proteins and lipids and inhibition of cellular functions. In response to oxidative stress, mitochondrial transmembrane potential dissipates, resulting in cytochrome c release and activation of metacaspases.

[New photo-induced effects of reactivation and protection of yeast cells under lethal UVB radiation].

Brief exposure of yeasts to low-intensity monochromatic light (400-730 nm) has revealed the effects of photoreactivation and photoprotection of the cells inactivated by medium wave UVB radiation (290-320 nm). The red spectral region with a maximum at 680 nm has been found to be the most active in the initiation of photoreactivation and photoprotection. It has been noted that, according to the regularities investigated, these processes differ fundamentally from the known processes of enzymatic photoreactivation and photoprotection, which have a spectral response limited by, respectively, blue (< 450 nm) and near (< 380 nm) UV light.

Biological photoreceptors of light-dependent regulatory processes.

Progress in understanding primary mechanisms of light reception in photoregulatory processes is achieved through discovering new biological photoreceptors, chiefly the regulatory sensors of blue/UV-A light. Among them are LOV domain-containing proteins and DNA photolyase-like cryptochromes, which constitute two widespread groups of photoreceptors that use flavin cofactors (FMN or FAD) as the photoactive chromophores. Bacterial LOV domain modules are connected in photoreceptor proteins with regulatory domains such as diguanylate cyclases/phosphodiesterases, histidine kinases, and DNA-binding domains that are activated by photoconversions of flavin.