Local and Systemic Functional Responses of Mouse Macrophages to Intravaginal Infection with Type 2 Herpes Simplex Virus and Vaccination.

Activity of cathepsin D and phagocytosis of macrophages from vaginal lavage fluid, peritoneal exudation, and spleen were studied in mice of sensitive (DBA/2) and resistant (BALB/c) lines after intravaginal infection with type 2 herpes simplex virus and vaccination. Activity of cathepsin D and intensity of phagocytosis (irrespective of the macrophage source) and their ratio in BALB/c mice in early terms after infection were close to the control levels taken as a unit. In DBA/2 mice, these parameters and their balance were shifted and changes in cathepsin D activity depended on the time after challenge.

Role of eicosanoids in regulation of macrophage phagocytic functions by platelet-activating factor during endotoxic shock.

We studied the role of eicosanoids in the regulation of macrophage phagocytic functions by products secreted in heterogeneous populations of macrophages and platelet-activating factor during endotoxic shock. Phagocytic activity depended on the metabolism of arachidonic acid in target macrophages and the ratio between its cyclooxygenase and lipoxygenase metabolites produced by heterogeneous populations of macrophages and affecting target cells. The regulatory effect of platelet-activating factor on phagocytosis was related to its interaction with products of the arachidonic acid cascade.

[Structural-functional changes in biomembranes during complications of diabetes mellitus and their pharmacological correction].

The decrease of sorbitol dehyidrogenase activity and the increase both of aldoso reductase activity and sorbitol concentration in aorta or eye lens under experimental diabetic angiopathy have been shown. The structural and functional alterations in erythrocytes membranes under angiopathy studied by spin- and luminesoense probes confirm the hypothesis on the major role of membrane pathology processes in the pathogenesis of diabetic mellitus. Pharmacological correction of diabetic angiopathy by glucophag or new antioxidant LBK-78 normalized the studied diversions on the membranes level.

[The hormone-metabolic changes in the diabetic angiopathy].

Biochemical, morphological and electron-microscopic readings of blood serum, liver, aorta and brain vessels, and in crystalline lens were investigated in intact and experimental animals during the process of experimental diabetes mellitus formation. The experimental diabetes mellitus was induced by L-adrenaline implementation and atherogenous load. Characteristics of lipid metabolism, peroxide lipid oxidation, sorbitol way of glucose metabolism, glucose and insulin levels conform to diabetes mellitus.