[Migraine in the real-life clinical practice: results of the observational study of diagnosis and treatment patterns in the urban population of the Russian Federation].

Objective: Analysis of real-life clinical practice data regarding the patterns of diagnosis and treatment of migraine in the Russian Federation.

Material And Methods: An observational real-world cohort study was conducted from January 2013 to December 2018 (over a 5-year period) to analyze the electronic database of 5 large private clinic chains in Moscow and St. Petersburg.

[Rubella virus genetic determinant of attenuation].

Vaccination is the most effective and available way to prevent Rubella. Presently, 9 vaccine strains were registered. Nevertheless, the molecular mechanisms of the attenuation were poorly elucidated for the rubella virus.

[Local flap technique].

The new local flap technique based on cutting out valve flaps combining with the principle of acute dermatension is presented in this article. The method is offered as alternative to plasty with transposed triangular flaps or Z-plasty and it allows to excise pathological scars, provides good esthetic results. On the basis of this technique some methods of eliminating post burn deformities of face, neck, mammary glands, extremities joints, hand and fingers are offered.

Quantification of measles, mumps and rubella viruses using real-time quantitative TaqMan-based RT-PCR assay.

In this study, a rapid quantitative method using TaqMan-based real-time reverse transcription-polymerase chain reaction (qPCR-RT) has been developed for estimating the titers of measles, mumps and rubella (MMR) viruses in infected cell culture supernatants. The qPCR-RT assay was demonstrated to be a specific, sensitive, efficient and reproducible method. For MMR viral samples obtained during MMR viral propagations in Vero cells at a different multiplicity of infection, titers determined by the qPCR-RT assay have been compared with estimates of infectious virus obtained by a traditional commonly used method for MMR viruses - 50% cell culture infective dose (CCID(50)) assay, in paired samples.