In vivo determination of transport and metabolism of deoxyglucose in intestinal tissues.

Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy-D-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in order to determine net rate of transport and phosphorylation of the material. The theoretical basis for calculating the rate constants of forward (kx1) and reverse (kx2) transport between plasma and tissue and also phosphorylation (kx3) and dephosphorylation (kx4) of 14C labeled deoxy-glucose (DG) was determined.