Sonographer-guided frozen embryo transfer vs ultrasound-assisted frozen embryo transfer - A randomised controlled study.

Background: The use of ultrasound to guide placement of the embryo during in vitro fertilisation (IVF) is important, but there are times where a good image cannot be obtained. Having a trained sonographer perform the ultrasound may therefore improve the success of embryo transfer.

Aim: To determine whether the routine use of a sonographer to guide embryo transfer is superior to standard care.

Safe CRISPR-Cas9 Inhibition of HIV-1 with High Specificity and Broad-Spectrum Activity by Targeting LTR NF-κB Binding Sites.

Viral latency of human immunodeficiency virus type 1 (HIV-1) has become a major hurdle to a cure in the highly effective antiretroviral therapy (ART) era. The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has successfully been demonstrated to excise or inactivate integrated HIV-1 provirus from infected cells by targeting the long terminal repeat (LTR) region. However, the guide RNAs (gRNAs) have classically avoided transcription factor binding sites (TFBSs) that are readily observed and known to be important in human promoters.

Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients.

The CRISPR/Cas9 system has been proposed as a cure strategy for HIV. However, few published guide RNAs (gRNAs) are predicted to cleave the majority of HIV-1 viral quasispecies (vQS) observed within and among patients. We report the design of a novel pipeline to identify gRNAs that target HIV across a large number of infected individuals.

Kansas Provider Report of Adolescent Vaccinations in Their Practice.

Background: Kansas falls consistently below average for adolescent vaccination of meningococcal (MCV), human papillomavirus (HPV), and influenza.

Methods: For this study, the members of Kansas Chapter of the American Academy of Pediatrics were emailed a confidential electronic survey soliciting their impressions of vaccination in their practice.

Results: Of 137 providers emailed, 61 (45%) completed the survey.

The Relationship of Personality Style and Attention Deficit Hyperactivity Disorder in Children.

Introduction: This study was to identify personality correlates of children with a diagnosis of Attention Deficit Hyperactive Disorder (ADHD). The Jungian Personality Type dimensions primarily considered were Sensing/Intuiting and Perceiving/Judging. A Sensing child is likely to be very present-centered.