Callus formation and plant regeneration in standard and microexplants from seedlings of barley (Hordeum vulgare L.).

Callus was induced in standard (1 mm) leaf base explants and in cross sections (microexplants) through the seedling axis from seedlings of Hordeum vulgare L.. Reduced callus formation was observed with increasing distance from the leaf base, and explants from the first and second leaves gave the best response.

The kallikrein-kinin system in the acutely-ill: (A) changes in plasma kininogen in acutely-ill patients. (B) the efficacy of pulmonary clearance of bradykinin.

These studies have indicated some quantitative aspects of the kallikrein kinin system in sepsis. While other investigators have noted the fall in plasma kininogen in patients with sepsis, e.g.

A high performance liquid chromatographic method for the determination of kinin releasing activity of kallikrein.

An HPLC method for measuring the in vitro kinin-forming activity of Kallikrein (K), is described. Kinins are formed by incubating K with partially purified bovine kininogen. An aliquot portion of the incubation solution is injected directly onto the chromatographic column (RP-18, 10 um, length 25 cm, 4 mm i.

Experimental studies on the influence of the kallikrein-kinin system on glucose utilization.

Our studies, together with the findings of other authors, indicate that kallikreins, via kinin release, play a role in glucose metabolism. It is most likely that the utilization of glucose is enhanced directly within the energy metabolizing cell itself but not indirectly by way of an insulin release in the pancreatic islets, since the in vitro studies on isolated islets of Langerhans did not reveal any effect of bradykinin on insulin secretion or insulin release. Further investigations are, however, necessary to clarify to what extent and in what way the kallikrein-kinin system is involved in causing the increased glucose utilization.