Background: The shape and microstructure of the human crystalline lens alter with ageing, and this has an effect on the optical properties of the eye. The aim of this study was to characterise the age-related differences in the morphology and transparency of the eye lenses of healthy subjects through the optical signal discontinuity (OSD) zones in optical coherence tomography (OCT) images. We also investigated the association of those changes with the optical quality of the eye and visual function.
View Article and Find Full Text PDFCataract is one of the common causes of visual impairment due to opacification of the crystalline lens. Increased intraocular scattering affects the vision of cataract patients by reducing the quality of the retinal image. In this study, an amplitude modulation-based scatter compensation (AM-SC) method is developed to minimize the impact of straylight on the retinal image.
View Article and Find Full Text PDFThe paper constitutes a concise study of light diffraction by two superposed ultrasonic waves of the frequency ratio 1:2, beyond the Raman-Nath diffraction regime. Relevant numerical simulations of light intensity distribution within separate diffraction orders are shown based on the Nth order approximation method (NOA). A comparison is given between generalized theoretical predictions (N=7) and simplified formulas of Mertens (N=2) [R.
View Article and Find Full Text PDFWe describe the phenomenon of a sudden decrease in the amplitude of photoacoustic signals arising from nanosecond laser pulse irradiation of biological samples, measured in vitro. Several dental enamel and chicken/turkey breast samples are examined. Moderate optical energy densities (i.
View Article and Find Full Text PDFSimple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc(TM) 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
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