[The synthesis of triterpenic amides based on 2,3-seco-1-cyano-19beta,28-epoxy-18alpha-olean-3-oic acid].

Novel 2,3-seco-triterpenic amides were prepared by the interaction of the chloride of 2,3-seco-l-cyano-19beta,28-epoxy-18alpha-oleane-3-oic acid with primary amines and synthetic and biogenic amino acids. A cytotoxic triterpenic conjugate with a residue of the ethyl ester of beta-alanine was found among the synthesized nitrogen-containing derivatives. Treatment with this conjugate in a concentration of 100 muM resulted in the 45.

Combined effect of cisplatin and lymphokine-activated killer cells on A549 cells of non-small cell lung cancer.

We studied the ability of lymphokine-activated killer cells to lyse A549 human non-small cell lung cancer cells after preincubation with cisplatin. Lymphokine-activated killer cells obtained after incubation of human blood lymphocytes with interleukin-2 were characterized by high expression of natural killer cell antigens and activation molecules. Lymphokine-activated killer cells produced potent cytotoxic effect on intact A549 cells and lysed tumor cells survived after treatment with cisplatin in concentrations of IC50 and IC30.

[New approaches to search for antitumoral compounds].

The authors discuss the present-day state of search for antitumoral compounds at Blokhin Russian Oncological Research Center and promising approaches including computer technologies as means of search for new anticancerous targets and drugs.

Selective antitumor activity of lymphokine-activated killer cells in vitro.

We compared cytotoxic activity of blood mononuclear leukocytes from healthy donors and lymphokine-activated killer cells generated from them towards tumor and normal cells. Lymphokine-activated killer cells exhibited higher (in comparison with blood mononuclear leukocytes) killer activity towards tumor cells. Lymphokine-activated cells and mononuclear leukocytes had no lytic effect on non-transformed eukaryotic cells.

[Using nutriceutics for occupational fluorosis prevention].

The article deals with pathogenesis of occupational fluorosis. Clinical studies demonstrated changes in bone mineral density among workers with 15-20 years of service. Experimental studies proved that occupational fluorosis is associated withcalcium dysbalance,bone tissue resorption and calcium washout.