[Preparative isolation of the glycosylated protein and the major core protein of bovine leukemia virus].

A technique for purifying intact bovine leukemia virus (BLV) and a procedure for preparing gag protein (p24) and envelope glycoprotein (gp 51) from intact BLV are described. The preparation method employs solubilization of BLV by n-octyl-beta-D-Glycopyranoside and sodium desoxycholate with following ultracentrifugation in a linear 10-60% sucrose gradient. The isolated components preserve their antigenicity.