[The histochemical detection of insulin in a culture of pancreatic endocrine tissue by using the pseudoisocyanine and immunofluorescence methods].

Histochemical pseudoisocyanin and immunofluorescent methods were employed to investigate the level of insulin in cultivated pancreatic islet tissue (PIT) using two techniques: 1) staining of isolated PIT, cultivated on mica sheets; 2) staining of cultivated PIT sections. Stained sections ensure better results of microscopic and photometric investigations. On the whole, the most optimum method for histochemical analysis of the state of cultivated PIT is the combination of the above two techniques.

[A histofluorimetric method of evaluating the insulin level of pancreatic islets].

Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.

[The ultrastructure of pancreatic beta cells in dithizone diabetes and its prevention by sodium diethyldithiocarbamate].

Administration of a nondiabetogenic complex-former-sodium diethyldithiocarbamate (SDEDTC) to rabbits prevented the appearance in beta-cells of the insular apparatus of histological and ultramicroscopic changes with the subsequent administration of a potent diabetogenic agent-ditisone. SDEDTC blocked the insular zinc and prevented ditisone from forming in the islets a complex with this metal producing a destructive action of beta-cells.