[A method of calculating the liposome membrane permeability using dialysis method].

A correct mode of calculating liposome membrane permeability determined with dialysis is proposed. The liposome membrane permeability is calculated with regard for the ion passage through two diffusion barriers: liposomal membrane and cellophane membrane. The asymmetrical ion distribution under equilibrium conditions is shown.

[Ouabain-insensitive K+ and Na+ fluxes in frog muscle].

Potassium and sodium fluxes in frog muscles incubated in K- and Na-free Mg2+ or choline-substituted Ringer solution were studied. Ouabain (10(-4) M) increased the potassium and decreased the sodium efflux. It was shown, that the increase of the potassium efflux was not due to changes in membrane electrical properties of inhibition of "back pumping" of potassium ions leaking out of the cells.

[Temperature characteristics of ouabain-insensitive sodium flux in frog muscle].

Temperature influence on ouabain-insensitive non-diffusion sodium efflux from the frog skeletal muscle loaded by sodium was studied. It is puzzling that the up-gradient sodium efflux supplied by enzymatic reaction is characterized by low temperature coefficient (Q10 = 1.3).

[Relation between ouabain-insensitive differential sodium flux and sodium concentrations in solution].

The dependence of net sodium ion flux on the external sodium concentration was studied under the inhibition of the sodium pump. A steady state balance of sodium ion fluxes occurred at external sodium concentration differing from the calculated one, therefore the existence of a non-diffusion component of sodium ion flux was evident. It was shown that the non-diffusion ouabain-insensitive sodium ion flux consisted of the sodium efflux not connected with chlorine efflux or potassium influx, and the sodium influx connected, perhaps, with potassium efflux.