Publications by authors named "G G Eveleth"

In this article, we address the issue of persistence of chromosome exchanges following acute in vitro exposure of rat peripheral blood to 137Cs. Irradiation occurred 24 hr after culture initiation, and metaphase chromosomes were prepared 2, 3, 4, and 5 days later. Chromosomes 1, 2, and 4 were painted in unique colors and scored for structural aberrations.

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We report a new physical configuration for the detection of perpendicular light scatter or fluorescence in flow cytometry when using a fluid stream in air. This configuration increases the signal-to-noise ratio and narrows the coefficient of variation for uniformly sized latex spheres when compared to using a microscope objective to collect such light. The new technique views the scattered light that is trapped within the optical waveguide that is naturally formed by the flow stream in air.

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Three human chromosome 2-specific clone libraries were constructed and characterized. Chromosome 2-specific cosmid and fosmid clone libraries were constructed using flow-sorted DNA from the monochromosomal hybrid cell line GM10826. The cosmid and fosmid libraries consist of 38,496 and 26,400 arrayed clones, respectively, with an average size of 40 kb.

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Using a high-speed flow cytometer/sorter, we constructed spot-blot "stamps" measuring 3.5 x 2.0 cm containing 21 separate human chromosome fractions.

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The speed and ease of human cytogenetic analysis has been greatly enhanced by the technique of fluorescence in situ hybridization (FISH). Non-radioactive fluorescently tagged complex DNA probes specific for individual chromosomes can be hybridized to conventionally obtained metaphase chromosome spreads. Several chromosomes may be "painted" concurrently by using combinations of different labeled probes.

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