A fluorescence polarization assay using recombinant protein for the detection of antibodies against pathogenic in bovine.

Background: Bovine tuberculosis (bTB) is a major bacterial disease that causes significant economic disruption across the globe.

Aims: Our study was based on using a fluorescence polarization assay (FPA) that used fluorescein-labeled ESAT-6 protein to detect antibodies in bovine serum.

Methods: The ESAT-6 protein was used in a FPA.

Patho-epidemiological and risk factor studies for detection of Neospora-associated abortion in cattle and buffaloes in Punjab, India.

The diagnosis of abortion in livestock is difficult. In this paper, Neospora caninumassociated abortion in cattle and buffaloes in India is reported for the first time. A total of 184 animals (cattle, n = 133; water buffaloes, n = 51) were randomly selected for seroprevalence studies from 28 farms with a history of abortion.

Evaluation of adenosine deaminase activity in serum of cattle and buffaloes in the diagnosis of bovine tuberculosis.

Background And Aim: Bovine tuberculosis (bTB) is a chronic bacterial disease of cattle caused by . bTB causes severe economic losses resulting from livestock deaths, chronic disease, and trade restrictions. Determination of serum levels of adenosine deaminase (ADA), an enzyme produced by monocytes/macrophages and lymphocytes, has been used in the diagnosis of human TB.

Cell free mitochondrial DNA in serum and milk associated with bovine mastitis: a pilot study.

Mastitis is inflammation of mammary gland affecting all the species of domestic animals. Fragments of the mitochondrial genome released from dying cells are considered surrogate markers of mitochondrial injury. We hypothesized that bovine mastitis would be associated with increased cell free mitochondrial DNA (mtDNA) content in serum and milk.

ISMap element targeted nested polymerase chain in the detection of subsp. in fecal samples of cattle and buffaloes.

Background And Aim: Johne's disease is chronic granulomatous enteritis which affects ruminants. There are many diagnostic approaches for the detection of subsp. (MAP) of which molecular detection methods using various elements are less time consuming and more accurate.