Publications by authors named "G Falero-Diaz"
Objective: Monocytes are innate immune cells that play a central role in inflammation, an essential component during neovascularization. Our recent publication demonstrated that ischemia training by 24 h unilateral occlusion of the femoral artery (FA) can modify bone marrow-derived monocytes (BM-Mono), allowing them to improve collateral remodeling in a mouse model of hindlimb ischemia. Here, we expand on our previous findings, investigating a potential systemic effect of ischemia training and how this training can impact BM-Mono.
View Article and Find Full Text PDFObjective: This study focuses on dose-response investigation using a codon-optimized and de novo-synthesized E-Selectin/AAV2 (E-Sel/AAV2) vector in preparation for Investigational New Drug enabling of subsequent clinical studies.
Background: Gene therapy is a potential solution for patients suffering from chronic limb-threatening ischemia. Understanding the dose for effective gene delivery is crucial for future Investigational New Drug-enabling studies.
Article Synopsis
- Critical limb ischemia (CLI) is a severe form of peripheral artery disease affecting a significant number of patients, with some unable to receive current treatments.
- The study used conditional knockout mouse models to investigate the role of endothelial c-Kit signaling in blood vessel formation during hindlimb ischemia, comparing these mice with control groups.
- Findings revealed that disabling c-Kit and its ligand SCF in endothelial cells led to improved limb blood flow and better endothelial barrier function, highlighting potentially harmful effects of c-Kit signaling in these processes.
Ischemic-Trained Monocytes Improve Arteriogenesis in a Mouse Model of Hindlimb Ischemia.
Arterioscler Thromb Vasc Biol
February 2022
Objective: Monocytes, which play an important role in arteriogenesis, can build immunologic memory by a functional reprogramming that modifies their response to a second challenge. This process, called trained immunity, is evoked by insults that shift monocyte metabolism, increasing HIF (hypoxia-inducible factor)-1α levels. Since ischemia enhances HIF-1α, we evaluate whether ischemia can lead to a functional reprogramming of monocytes, which would contribute to arteriogenesis after hindlimb ischemia.
View Article and Find Full Text PDFMycobacterium tuberculosis (Mtb) is a pathogenic bacterium that causes tuberculosis (TB). This contagious disease remains a severe health problem in the world. The disease is transmitted via inhalation of airborne droplets carrying Mtb from TB patients.
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