[Early changes in template DNA structure following glucocorticoid injection].

The structure of chromatin is changed at early stages of glucocorticoid hormone interaction with rat hepatocytes. These changes consist in: a) increase of actidine orange binding in rate liver nuclei after injection of the hormone; b) decrease of the number of sites in chromatin which are sensitive to nuclease S1; c) inhibition of the nuclei capacity for DNA synthesis in the presence of E. coli DNA polymerase and d) increase of molecular weight of DNA fragments.

[Binding of glucocorticoid-receptor complexes with the acceptor zones of nuclei and effect of glucocorticoids on RNA synthesis in hormone-sensitive and hormone-resistant cell populations].

The binding of free radioactive glucocorticoid and the glucocorticoid-receptor complex to rat liver nuclei was studied in vitro. The binding is non-saturated and independent of preliminary injection of the "cold" hormone. In the course of DNA hydrolysis the amount of the radioactive hormone bound to the chromatin moiety in vivo remains practically unchanged relatively to the initial radioactivity of the protein.