Development and antitumor activity of a BCL-2 targeted single-stranded DNA oligonucleotide.

PNT100 is a 24-base, chemically unmodified DNA oligonucleotide sequence that is complementary to a region upstream of the BCL-2 gene. Exposure of tumor cells to PNT100 results in suppression of proliferation and cell death by a process called DNA interference. PNT2258 is PNT100 that is encapsulated in protective amphoteric liposomes developed to efficiently encapsulate the PNT100 oligonucleotide, provide enhanced serum stability, optimized pharmacokinetic properties and antitumor activity of the nanoparticle both in vivo and in vitro.

Amphoteric liposomes enable systemic antigen-presenting cell-directed delivery of CD40 antisense and are therapeutically effective in experimental arthritis.

Objective: Mediation of RNA interference by oligonucleotides constitutes a powerful approach for the silencing of genes involved in the pathogenesis of inflammatory disease, but in vivo application of this technique requires effective delivery to immune cells and/or sites of inflammation. The aim of the present study was to develop a new carrier system to mediate systemic administration of oligonucleotides to rheumatoid arthritis (RA) joints, and to develop an antisense oligonucleotide (ASO)-based approach to interfere with CD40-CD154 interactions in an experimental model of RA.

Methods: A novel liposomal carrier with amphoteric properties, termed Nov038, was developed and assessed for its ability to systemically deliver an ASO directed against CD40 (CD40-ASO).

Early intestinal changes following abdominal radiotherapy comparison of endpoints.

Purpose: To compare tests for intestinal function with clinical scores after abdominal irradiation.

Patients And Methods: At the Department of Radiotherapy, Erfurt, Germany, intestinal changes were studied in 91 patients receiving abdominal radiotherapy between 1992 and 1996. Conventional fractionation (1.

Effect of dose and dose-distribution in damage to the kidney following abdominal radiotherapy.

Purpose: To define the effects of abdominal radiotherapy on the kidney with regard to dose-distribution parameters.

Materials And Methods: Ninety-one patients with abdominal radiotherapy were included in the study, and the minimum follow-up was 1 year. Conventional fractionation, 5 x 1.

[Functional esophageal scintigraphy (99mTc-DTPA)1.2. Presentation of a method for diagnosis of effective deglutition in patients with oral and hypopharyngeal tumors].

Background: Today more than 60% of 65-year-olds complain of dysphagia or odynophagia. The aim of this study was to use esophageal scintigraphy as a means of estimating quantitative, reproducible, and comparable parameters of the swallowing ability in patients with tumors of the oropharynx and hypopharynx before and after surgery. Another group of patients suffering from larynx tumors was also examined prior to and following laryngectomy.