Enhancing Anti-Tumorigenic Efficacy of Eugenol in Human Colon Cancer Cells Using Enzyme-Responsive Nanoparticles.

This study is focused on the selective delivery and release of the plant-based anticancer compound eugenol (EUG) in colorectal cancer cells (CRC). EUG is an apoptotic and anti-growth compound in diverse malignant tumors, including CRC. However, EUG's rapid metabolization, excretion, and side effects on normal cells at higher dosages are major limitations of its therapeutic potential.

Acid-Triggered Release of Eugenol and Fluoride by Desensitizing Macro- and Nanoparticles.

The modern dentifrice industry needs non-toxic materials able to adhere to dentin, occlude dentinal tubules, hold pharmacons at the surface of dentin, and release them on demand to the location the tooth needs them most. Novel dental materials loaded with eugenol or fluoride-ions examined for the release of the pharmacon in an aqueous suspension efficiently adhere to the surface of human dentin and occlude dentinal tubules as evidenced by Scanning Electron Microscopy (SEM). Ultraviolet-visible (UV-vis) absorption spectroscopy and a fluoride-selective electrode quantified the release of pharmacons.

Expression of Specific Alleles of Zinc-Finger Transcription Factors, and , and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations.

Two genes, and , encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of and one in , among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for and , one SNP in each gene, and in each of two populations, showing simple Mendelian segregation.

Nanoscale mineralization of cell-laden methacrylated gelatin hydrogels using calcium carbonate-calcium citrate core-shell microparticles.

Conventional biomaterials developed for bone regeneration fail to fully recapitulate the nanoscale structural organization and complex composition of the native bone microenvironment. Therefore, despite promoting osteogenic differentiation of stem cells, they fall short of providing the structural, biochemical, and mechanical stimuli necessary to drive osteogenesis for bone regeneration and function. To address this, we have recently developed a novel strategy to engineer bone-like tissue using a biomimetic approach to achieve rapid and controlled nanoscale mineralization of a cell-laden matrix in the presence of osteopontin, a non-collagenous protein, and a supersaturated solution of calcium and phosphate medium.