Correction of Mitochondrial Enzyme Activities in the Skeletal Muscles of Old Rats in Response to Addition of Olive Oil to the Ration.

Activities of mitochondrial electron transport chain enzymes NADH-CoQ oxidoreductase (complex I), cytochrome C-oxidase (complex IV), and citrate synthase were measured by spectrophotometry in m. quadriceps femoris homogenate from old rats receiving olive oil with the ration. Reduced activities of complexes I and IV in old animals were restored to the level of young animals after 6-week consumption of olive oil.

[Alteration of brown adipocyte Ca2+ responses in culture by adrenergic activation].

Thermogenic capability of brown adipose tissue is controlled by norepinephrine. Interaction of norepinephrine with adipocyte at- and P3-adrenergic receptors results in the increase of Ca2+ and cAMP concentrations. The [Ca2+]i changes initiated by norepinephrine and selective agonists of alpha1- and beta-adrenergic receptors, cirazolin and isoproterenol, were recorded in single cells of primary culture on the 1st, 3rd and 6th days in vitro.

[Biological activity of water-soluble nanostructures of dihydroquercetin with cyclodextrins].

The biological properties of dihydroquercetin (DHO) modified by including it into the ring of beta-cyclodextrin (beta-CD) to give it more water-soluble properties have been investigated. It was shown that the peroral administration of the DHQ/beta-CD complex provides a long increase of DHQ concentration in rat blood (up to 7.5 h), and, unlike pure DHQ, the complex does not accumulate in the liver.

Dietary supplementation of old rats with hydrogenated peanut oil restores activities of mitochondrial respiratory complexes in skeletal muscles.

The effect of dietary supplementation of old rats (26-33 months) with hydrogenated peanut oil on the activity of mitochondrial enzymes in skeletal muscles has been studied. The activities of NADH-coenzyme Q1 oxidoreductase, cytochrome c oxidase, and citrate synthase were determined spectrophotometrically in muscle homogenates. The activities of respiratory complexes I and IV were shown to significantly decrease with the age compared to the activity of the same enzymes in young animals, while the activity of citrate synthase was virtually unchanged.

[Comparative analysis of Ca(2+)-signalling in brown preadipocytes of ground squirrel Spermophillus undulatus and mouse].

Analysis of the slow Ca(2+)-responses of brown preadipocytes of ground squirrel Spermophillus undulatus and mouse was carried out. The mouse brown preadipocytes demonstrated low but prominent responses to noradrenalin with the maximum at 3 and 10 microM being the less effective. The ground squirrel brown preadipocytes practically did not practically respond to 10 nM-10 microM, whereas 30-600 microM noradrenalin was able to raise intracellular [Ca2+]i up to 600 nM with 300 microM agonist being the most effective.