Induction of callus formation from difficile date palm protoplasts by means of nurse culture.

We report here for the first time callus formation from protoplasts in date palm (Phoenix dactylifera L.). Protoplasts were isolated from young leaves of offshoots and embryogenic calli in Deglet nour and Takerboucht genotypes.

Production of potato intraspecific somatic hybrids with improved tolerance to PVY and Pythium aphanidermatum.

Somatic hybridization can be an interesting alternative for the selection of heterozygous and vigorous potato plants through combination of dihaploid genomes. The resulting hybrids can harbour interesting characters and thus can be used in agriculture if they are in agreement with agronomic criteria. In this report, we used an intraspecific somatic hybridization technique for the production of tetraploid potato lines.

Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L.

Solanum aethiopicum is reported to carry resistance to bacterial wilt disease caused by Ralstonia solanacearum, which is one of the most important diseases of eggplant (Solanum melongena). These two species can sexually be crossed but the fertility of their progeny is very low. In order to transfer the resistance and improve the fertility, somatic hybrids between S.

Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja.

Somatic hybrid plants were produced after protoplast electrofusion between a dihaploid potato, cv. BF15, and a wild tuber-bearing relative, Solanum phureja, with a view to transferring bacterial wilt resistance into potato lines. A total of ten putative hybrids were selected.

[Cortex-wall connections in the apical cell of Sphacelaria].

The apical cell of Sphacelaria (Fucophyceae) exhibits a permanent polarized organization throughout asymmetric divisions. The apex organization was studied by immunolocalization of tubulin, vitronectin, alpha-actinin and beta 1 integrin. Microfilaments were stained directly by fluorescein phalloidin.