Phosphatidylserine liposomes induce a phagosome acidification-dependent and ROS-mediated intracellular killing of in human macrophages.

(Mab) is an opportunistic nontuberculous mycobacterium responsible of difficult-to-treat pulmonary infections in vulnerable patients, such as those suffering from Cystic Fibrosis (CF), where it represents a major cause of morbidity and mortality. Additionally, due to the intrinsic extensive antimicrobial resistance spectrum displayed by this species and the side effects reported for some available antibiotics, the therapeutic management of such infections remains extremely difficult. In the present study, we show that phosphatidylserine liposomes (PS-L) enhance intracellular mycobacterial killing of Mab infected human macrophages with functional or pharmacologically inhibited cystic fibrosis conductance regulator (CFTR), by a mechanism involving phagosome acidification and reactive oxygen species (ROS) production.

Analysis of Four New Phages and Modeling of a Hyaluronidase Catalytic Domain from .

Phage therapy (PT), as a method to treat bacterial infections, needs identification of bacteriophages targeting specific pathogenic host. , a Gram-positive coccus resident in the human gastrointestinal tract, may become pathogenic in hospitalized patients showing acquired resistance to vancomycin and thus representing a possible target for PT. We isolated four phages that infect and characterized them by host range screening, transmission electron microscopy, and genome sequencing.

A Phage Therapy Model for the Prevention of pv. Infection of Kiwifruit Plants.

Great efforts have been made with chemicals and pesticides to contain the spread of pv. () responsible for kiwifruit canker. Unfortunately, only partial results were obtained for this bacterial pandemic, and alternative remedies were proposed to avoid soil pollution and the onset of antibiotic resistance.

Evidence of Another Anthropic Impact on from the Lesser Antilles: The Presence of Antibiotic Resistant Enterobacteria.

The improper use of antibiotics by humans may promote the dissemination of resistance in wildlife. The persistence and spread of acquired antibiotic resistance and human-associated bacteria in the environment, while representing a threat to wildlife, can also be exploited as a tool to monitor the extent of human impact, particularly on endangered animal species. Hence, we investigated both the associated enterobacterial species and the presence of acquired resistance traits in the cloacal microbiota of the critically endangered lesser Antillean iguana (), by comparing two separate populations living in similar climatic conditions but exposed to different anthropic pressures.