Imaging the structural connectome with hybrid MRI-microscopy tractography.

Mapping how neurons are structurally wired into whole-brain networks can be challenging, particularly in larger brains where 3D microscopy is not available. Multi-modal datasets combining MRI and microscopy provide a solution, where high resolution but 2D microscopy can be complemented by whole-brain but lowresolution MRI. However, there lacks unified approaches to integrate and jointly analyse these multi-modal data in an insightful way.

An open resource combining multi-contrast MRI and microscopy in the macaque brain.

Understanding brain structure and function often requires combining data across different modalities and scales to link microscale cellular structures to macroscale features of whole brain organisation. Here we introduce the BigMac dataset, a resource combining in vivo MRI, extensive postmortem MRI and multi-contrast microscopy for multimodal characterisation of a single whole macaque brain. The data spans modalities (MRI and microscopy), tissue states (in vivo and postmortem), and four orders of spatial magnitude, from microscopy images with micrometre or sub-micrometre resolution, to MRI signals on the order of millimetres.

Individual Differences in the Alignment of Structural and Functional Markers of the V5/MT Complex in Primates.

Extrastriate visual area V5/MT in primates is defined both structurally by myeloarchitecture and functionally by distinct responses to visual motion. Myelination is directly identifiable from postmortem histology but also indirectly by image contrast with structural magnetic resonance imaging (sMRI). First, we compared the identification of V5/MT using both sMRI and histology in Rhesus macaques.

Influenza A viruses isolated from migrating ducks in Oklahoma.

Nine type A influenza viruses were isolated from migrating and wintering ducks in Oklahoma in 1976-77. Antigenic classification of the viruses isolated revealed three different subtypes: Hav1 Nav2, Hws N1, and Hav6 N2. Transmission of influenza viruses from the wild ducks to sentinel birds (McGraw mallards) on the same lakes was not detected.

Structural changes associated with type-A influenza in Mallard duck tracheal organ culture.

Mallard duck tracheal organ cultures were used to study structural changes associated with infection with type-A influenza (A/Turkey/WIS/68) (H9N2) at the light-microscope and electron-microscope levels. Light-microscope changes in infected organ culture were cytoplasmic vacuolization, nuclear swelling, reduction in ciliated epithelium, and sloughing of epithelial cells. Ultrastructural changes included the loss of cilia and microvilli, distortion and swelling of cellular organelles, breakdown of intercellular junction, and apparent phagocytosis of the ciliated epithelium.