Publications by authors named "G Cecilia Luvoni"
Cryopreservation of canine ovarian tissue by slow freezing and vitrification: Evaluation of follicular morphology and apoptosis rate.
Theriogenology
December 2024
Article Synopsis
- The study evaluated two methods of cryopreserving canine ovarian tissue—vitrification and slow freezing—by examining their effectiveness and differences based on follicular type.
- Twenty-eight ovaries from 14 anoestrus bitches were used, divided into three groups for preservation (vitrification, slow freezing, and fresh control) with specific solutions and techniques applied for each.
- Results showed that slow freezing led to significantly more intact follicles and lower apoptosis rates compared to vitrification, indicating it is a more effective method for preserving viable ovarian tissue in dogs.
Global contraction of biodiversity pushed most members of Felidae into threatened or endangered list except the domestic cat (Felis catus) thence preferred as the best model for conservation studies. One of the emerging conservation strategies is vitrification of ovarian tissue which is field-friendly but not yet standardized. Thus, our main goal was to establish a suitable vitrification protocol for feline ovarian tissue in field condition.
View Article and Find Full Text PDFOvarian tissue vitrification is associated with multiple events that promote accumulation of ROS (reactive oxygen species) which culminate in follicular apoptosis. Thus, this study was aimed at evaluating the role of melatonin in vitrification and culture of feline (Felis catus) ovarian tissue. In phase 1, domestic cat ovaries were fragmented into equal circular pieces of 1.
View Article and Find Full Text PDFFixatives and fixation protocol have a profound effect on both the morphology and epitope sensitivity of ovarian tissue, which hampers accurate ovarian tissue evaluation. We aimed to establish the most suitable fixation protocol for feline () ovarian tissue. Fragments (1.
View Article and Find Full Text PDFIn Brief: Ovarian tissue cryopreservation and culture provide an option for fertility preservation without tissue grafting, but need optimization. This study reveals that vitrified bovine ovarian tissue can be cultured on agarose gel and maintain follicle morphology, low activation, and low apoptosis.
Abstract: Ovarian tissue preservation is hitherto a promising fertility insurance option for precious animals.