First-in-human study of TK-positive oncolytic vaccinia virus delivered by adipose stromal vascular fraction cells.

Background: ACAM2000, a thymidine kinase (TK)-positive strain of vaccinia virus, is the current smallpox vaccine in the US. Preclinical testing demonstrated potent oncolytic activity of ACAM2000 against several tumor types. This Phase I clinical trial of ACAM2000 delivered by autologous adipose stromal vascular fraction (SVF) cells was conducted to determine the safety and feasibility of such a treatment in patients with advanced solid tumors or acute myeloid leukemia (AML).

Circulating FSH, LH, GH, testosterone, TSH, T3, T4, prolactin and insulin in boys during puberty.

The dynamics of the serum FSH, LH, GH, T, TSH, T3, T4, prolactin (PRL) and insulin levels was studied in 50 healthy boys aged between 8 and 18 years, in correlation with the development of the gonad (sexualization) assessed through the five-stage classification (G I-G V) proposed by Tanner. The results reveal the role of gonadotropins (especially LH) and testosterone in the growth and development of the child and especially in the sexualization process through a progressive increase from stage GI to GV. In stage GV, LH and T reach the values of an adult.