[Histochemical assessment of immunoreactivity of thyroid peroxidase and its correlation with biochemical activity].

Background: Peroxidase content has been recently evaluated in normal thyroid and in different thyroid disorders by biochemical, histochemical, ultrastructural and immunocytochemical methods Nevertheless immunocytochemical detection of thyroid peroxidase in thyroid samples conventionally processed for histology hes never been done using a commercially available antibody, neither its correlation with the biochemical activity on adjacent samples.

Methods: In this study we have analyzed normal thyroid tissue (3 patients), follicular adenoma (2 patients) and multinodular goiter (2 patients) conventionally processed for histology and stiined by immunocytochemistry (Avidin Biotin System) using a polyclonal (rabbit) antibody for horseradish peroxidase (Serotec). Biochemical assay was performed on adjacent samples according to Hosoya method.

[Subcutaneous emphysema. Complication associated with pneumoperitoneum during videolaparoscopic cholecystectomy].

Diagnostic and therapeutic laparoscopy is a safe procedure, which, however, is not without complications. The rare occurrence of subcutaneous emphysema, as a consequence of pneumoperitoneum, following laparoscopic cholecystectomy, is reported. The mechanism for the development of this complication and its management are discussed.

[Post-partum vesicovaginal fistula: abdominal muscle strip treatment].

Background: The Vesico-Vaginal Fistula (VVF) very often occur in tropical countries, but their treatment is usually not correct.

Methods: A surgical treatment was carried put on 68 patients affected by VVF from March 1986 to December 19997 in the Nazareth Hospital (Nairobi). Their mean age was 22 years old; 27 patients (39.

[Arteriovenous fistulas for hemodialysis. Experience in 366 cases].

The surgical techniques adopted in performing 366 a-v fistulas for hemodialysis in 346 patients over ten years' experience are presented. A survey of the results obtained is then made as well as the evaluation of the same, based upon functional and clinical parameters. The technical details sometimes adopted, the occlusions and the principles drawn out for personal experience in this field are described.