Publications by authors named "G Cagney"
Despite the importance of grains and legumes in the human diet, little is known regarding peptide release and the temporal changes of protease activities during seed germination. LC/MS-MS peptidomic analysis of two cultivars of germinating chickpea followed by computational analyses indicated cleavage dominated by proteases with a single position preference (mainly before (P1) or after cleavage (P1'): L at P2 (cysEP-like); R or K at P1 (vignain-like), N or Q at P1 (legumain-like); and previously unidentified K, R, A and S at P1'; A at P2'). While P1 N cleavages were relatively constant, P1' K/R preferences were high in soaked garbanzo (kabuli) seeds, declined by four days, and returned at six days, but were much rarer in the brown (desi) cultivar.
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- The study investigated how the protein expression of NT2 embryonal carcinoma cells changes when treated with retinoic acid, indicating differentiation from a pluripotent state.
- Mass spectrometry was used to analyze over 5,000 proteins, revealing significant alterations in chromatin proteins and the regulation of epigenetic pathways during this process.
- The research found a connection between cathepsin proteases and histone modifications, showing that inhibiting these proteases decreases histone H3 clipping, which is important for gene expression changes linked to cell differentiation.
The study investigates the effect of conventional and novel extraction techniques on the protein extraction yield from bitter gourd seeds (Momordica charantia). Ultrasound assisted-extraction (UAE) treatment for 30 min at 4 °C using a 20 kHz ultrasound probe resulted in the highest extraction yield of crude proteins. After purification, 9.
View Article and Find Full Text PDFPseudomonas putida KT2440 is an important bioplastic-producing industrial microorganism capable of synthesizing the polymeric carbon-rich storage material, polyhydroxyalkanoate (PHA). PHA is sequestered in discrete PHA granules, or carbonosomes, and accumulates under conditions of stress, for example, low levels of available nitrogen. The pha locus responsible for PHA metabolism encodes both anabolic and catabolic enzymes, a transcription factor, and carbonosome-localized proteins termed phasins.
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- - Psychosis risk prediction in psychiatry is challenging, and a study aimed to evaluate whether a specific proteomic model could accurately predict the transition to psychosis in individuals at clinical high risk, using plasma samples from three cohorts totaling 754 participants.
- - The study found that only 20.4% of participants developed psychosis over about 4.4 years, and the prediction model had poor accuracy (C-statistic: 0.51), suggesting that the proteomic factors didn't reliably predict outcomes.
- - Some proteins, like Complement C8B and LRG1, showed weak associations with psychosis transition but did not reach significant levels when correcting for multiple comparisons, indicating that previous claims based on small sample sizes should