From banks to burrows: Habitat preferences and nesting behaviours of platypuses in the Snowy River.

Platypuses are a unique freshwater mammal native to eastern Australia. They are semi-aquatic, predominantly nocturnal, and nest in burrows dug into the banks of waterbodies. Quantifying nesting burrow characteristics is challenging due to the species' cryptic nature.

River regulation and climate change reduce river flows to major Australian floodplain wetland.

Freshwater ecosystems, including rivers and floodplain wetlands, face severe stress from unsustainable water resources development, with climate change exerting further pressure. This study compares the relative effects of river regulation and projected climate change on river flows to the semi-arid Lowbidgee Floodplain (3250 km), the largest wetland ecosystem on the heavily regulated Murrumbidgee River, Australia's second longest river, within the Murray-Darling Basin. We modelled annual natural streamflow in the lower Murrumbidgee River before major dam constructions and water diversions (1890-1927), linking river flows to runoff from the upper Murrumbidgee catchment.

Fragmentation by major dams and implications for the future viability of platypus populations.

The evolutionarily unique platypus (Ornithorhynchus anatinus) has experienced major declines and extinctions from a range of historical and recent interacting human-mediated threats. Although spending most of their time in the water, platypuses can move over land. Nevertheless, uncertainties remain whether dams are barriers to movement, thus limiting gene flow and dispersal, essential to evolution and ecology.

Using DNA metabarcoding as a novel approach for analysis of platypus diet.

Platypuses (Ornithorhynchus anatinus) forage for macroinvertebrate prey exclusively in freshwater habitats. Because food material in their faeces is well digested and mostly unidentifiable, previous dietary studies have relied on cheek pouch assessments and stable isotope analysis. Given DNA metabarcoding can identify species composition from only fragments of genetic material, we investigated its effectiveness in analysing the diet of platypuses, and to assess variation across seasons and sexes.

Seasonal and geographic variation in packed cell volume and selected serum chemistry of platypuses.

Platypuses (Ornithorhynchus anatinus) inhabit the permanent rivers and creeks of eastern Australia, from north Queensland to Tasmania, but are experiencing multiple and synergistic anthropogenic threats. Baseline information of health is vital for effective monitoring of populations but is currently sparse for mainland platypuses. Focusing on seven hematology and serum chemistry metrics as indicators of health and nutrition (packed cell volume (PCV), total protein (TP), albumin, globulin, urea, creatinine, and triglycerides), we investigated their variation across the species' range and across seasons.