The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew.

Powdery mildew poses a significant threat to global wheat production and most cloned and deployed resistance genes for wheat breeding encode nucleotide-binding and leucine-rich repeat (NLR) immune receptors. Although two genetically linked NLRs function together as an NLR pair have been reported in other species, this phenomenon has been relatively less studied in wheat. Here, we demonstrate that two tightly linked NLR genes, RXL and Pm5e, arranged in a head-to-head orientation, function together as an NLR pair to mediate powdery mildew resistance in wheat.

Machine learning-driven identification of critical gene programs and key transcription factors in migraine.

Background: Migraine is a complex neurological disorder characterized by recurrent episodes of severe headaches. Although genetic factors have been implicated, the precise molecular mechanisms, particularly gene expression patterns in migraine-associated brain regions, remain unclear. This study applies machine learning techniques to explore region-specific gene expression profiles and identify critical gene programs and transcription factors linked to migraine pathogenesis.

Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression.

Tea plant can enrich a large amount of fluorine (F) in the cell wall of its mature leaves, thus posing the risk of excessive intake of F for tea consumers. This study investigated the effect of foliar calcium (Ca) application (0.05-1 mM) on F accumulation in tea plant leaves by analyzing the association of F with cell wall materials, pectin methylesterification structure, and cell wall genes.

Designing a microbial factory suited for plant chloroplast-derived enzymes to efficiently and green synthesize natural products: capsanthin and capsorubin as examples.

Specific cellular microenvironment, multi-enzyme complex and expensive essential cofactor make the biological manufacturing of plant chloroplast natural products (PCNPs) extremely challenging. The above difficulties have hampered the biosynthesis of capsanthin and capsorubin in the past 30 years. Here, we take capsanthin and capsorubin as examples to design an innovative microbial factory to promote the heterologous synthesis of PCPNs.

Intraoperative sensitization in trigeminal region caused by postherpetic neuralgia: a case report.

Background: Interventional therapy of trigeminal neuropathic pain has been well documented; however, intraoperative monitoring and management of pain hypersensitivity remains barely reported, which may pose a great challenge for pain physicians as well as anesthesiologists.

Case Presentation: A 77-year-old Han Chinese male, who suffered from severe craniofacial postherpetic neuralgia, underwent pulsed radiofrequency of trigeminal ganglion in the authors' department twice. The authors successfully placed a radiofrequency needle through the foramen ovale during the first procedure with local anesthesia and intravenous sedation (dexmedetomidine).