The close correlation between ceramide accumulation and cardiolipin content decrease in cardiomyocytes, mitochondrial dysfunction and heart failure was well documented. Taking into account that ceramide can alter the cardiolipin metabolism the purpose of the present study was to determine the role of ceramide in age-dependent decline of cardiolipin content in rat heart tissue. In the present work the puberty young, adult and old (3-, 12-, 24-, 30- and 34-month-old) Wistar male rats were used.
View Article and Find Full Text PDFOld age-associated pathologies usually coincide with altered sphingolipid metabolism. In the present article, the role of acid sphingomyelinase (ASMase) in the age-dependent changes of sphingomyelin (SM) and ceramide contents in the tissues has been investigated by means of ASMase inhibitors, imipramine and zoledronic acid. It has been determined that ceramide content and ceramide/SM ratio increased, while SM level decreased in the heart, liver, blood serum and skeletal muscles of 24-month old rats in contrast to 3-month old animals.
View Article and Find Full Text PDFAge peculiarities of the calorie-restricted diet effects on the contents biologically active sphingo- and glycerolipids were studied in the heart, liver and brain of 3- and 24-month-old rats. Rats were either kept on the ad libitum diet or on a calorie restricted diet (70-80% reduction in total calories) without reduction in essential nutrients. It has been determined that calorie restricted diet decreased the ceramide, sphingomyelin, cardiolipin and phosphatidic acid levels in the all investigated tissues of the rats.
View Article and Find Full Text PDFBecause reduction of sphingomyelin and ceramide accumulation in aging are associated with the development of a wide variety of pathologies, finding the ways of normalizing the sphingolipid exchange is becoming actual. As the ASM is a key enzyme of the sphingolipid exchange, in the present study we have investigated the effect of an ASM inhibitor imipramine on the ceramide and sphingomyelin content in the tissues of old rats. The introduction of imipramine elevates the sphingomyelin level and reduces the ceramide/sphingomyelin ratio in the liver, heart and brain of old rats.
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