Corticosteroid activity, in vitro, in interrenal tissue of Atlantic salmon (Salmo salar) parr. 2. Comparative synthetic profiles from Nova Scotian stocks.

Differences observed in autoradiogram profiles and isotope ratios of corticosteroid metabolites (such as cortisol), in vitro, in interrenal activity between and within hatchery reared salmon (Salmo salar) parr stocks were not observed in corresponding wild stocks. Wild fish from nine Nova Scotian rivers (Annapolis, Black, East, Gold, LaHave, Medway. Mushamush, Salmon, and Stewiacke Rivers), sampled during May to December all showed similar corticosteroid profiles.

Corticosteroid activity, in vitro, in interrenal tissue of Atlantic salmon (Salmo salar) parr. 1. Synthetic profiles.

Fresh interrenal tissue of Atlantic salmon (Salmo salar) parr transformed [3H]pregnenolone and [14C]progesterone, in vitro, to double-labeled cortisol and corticosterone with yields of less than 3% 3H and 5% 14C at 0-5 degrees, 8-10 degrees, and 22-24 degrees over various incubation periods. Incubations at 22-24 degrees gave the highest yields. A time-yield study at 22-24 degrees for 0.

In vitro biosynthesis of 17 alpha,20 alpha,20 beta-dihydroxy-4-pegnen-3-one by the ovaries, testes, and head kidneys of the Atlantic salmon Salmo salar.

Ovaries, testes, and head kidneys of sexually mature Atlantic salmon, Salmo salar, biosynthesized 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-diOHP) from equimolar amounts of [3H]pregnenolone plus [4-14C]progesterone in vitro. The 3H:14C isotope ratios of steroid metabolites indicated that the biosynthetic pathways to 17 alpha,20 beta-diOHP in the testes differed from those observed in the ovaries and head kidneys. [4-14C]Progesterone appeared to be the principal precursor of 17 alpha,20 beta-diOHP in the testes, whereas both precursors were efficiently biotransformed to 17 alpha,20 beta-diOPH in the ovaries and head kidneys.

A simple, rapid, and precise direct radioimmunoassay method for 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one in salmon plasma.

This report describes a radioimmunoassay method for 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-diOHP) in the blood plasma of Atlantic salmon (Salmo salar). 3H-labeled 17 alpha,20 beta-diOHP was synthesized from 17 alpha-[3H]hydroxy-4-pregnen-3-one (17 alpha-OHP) by enzymatic reduction of the C-20 oxo group and the simultaneous oxidation of coenzyme-reduced nicotinamide adenine dinucleotide. Antiserum to 17 alpha,20 beta-diOHP-3-CMO-BSA exhibited high specificity: all steroids tested against the antiserum gave less than 1% cross-reactivity at 50% displacement.

Isolation and identification of testosterone from the serum and testes of the American lobster (Homarus americanus).

Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its acetate derivative. The concentrations of testosterone were determined by high-performance liquid chromatography, by a double-isotope derivative assay, and by radioimmunoassay.