In the present study, next generation sequencing was employed to identify and explore the differential expression profiles of microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) of crossbred ( x ) and Vechur () cattle in response to the bacterial endotoxin-lipopolysaccharide (LPS). The PBMCs from adult apparently healthy female crossbred cows and Vechur cattle, a native cattle breed of Kerala, India were stimulated with 10 μg/mL of LPS for 6 h. Among the differentially expressed miRNAs, the expression of 13 miRNAs showed statistically significant up regulation while, significant decrease in the expression of 15 miRNAs was noticed in LPS treated PBMCs of Vechur cattle compared to crossbred cows.
View Article and Find Full Text PDFIn the present scenario of research, the recycling of inexpensive widely available agricultural waste/biowaste to activate carbon (AC) and procurement of value-added product has significant impact on energy storage systems, particularly in Electrochemical double layer capacitors (EDLCs). Herein, we report the production of KOH activated carbons from different biomass sources such as stem (MAC), seed oil extract cake (PPC), stem (CCC) and floss (ASC) for the said purpose. Initially, the biomass materials were pyrolyzed at 550 °C and then activated with KOH at 800 °C.
View Article and Find Full Text PDFHypoxia-inducible factor (HIF)-1α is a transcription factor stabilized by hypoxia by inducing or suppressing the homeostatic regulatory gene expression, enabling tissues and cells to survive despite fluctuations in environmental circumstances. As the name implies, hypoxia-inducible factor-1 is secreted not only as a cellular response to hypoxia but also in heat stress and oxidative stress. The goal of this work was to determine the molecular characterisation of the gene coding region as well as the differences in HIF-1αprotein primary structure between Vechur cattle and other cattle breeds in the online databases.
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