Publications by authors named "G B Klafke"

Background: One bioassay for detecting acaricide resistance in livestock ticks is the adult immersion test (AIT), wherein engorged ticks are briefly immersed into a solution of a particular acaricidal compound and examined for mortality, their egg-laying capacity and offspring hatchability in vitro. Usually, the recommended label dose or an established discriminating dose of an acaricide is used to determine high mortality (≥ 95%) of susceptible tick strains. Such a test intends to detect the presence of resistance in a tick population.

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  • Bovine babesiosis, caused by Babesia bovis and Babesia bigemina, significantly impacts livestock and is often treated with imidocarb in areas lacking vaccines.
  • The study involved 15 heifers, with 12 receiving imidocarb and all exposed to ticks to evaluate the development of humoral immunity through seroconversion.
  • Results showed that after multiple treatments, a majority of the treated heifers did develop antibodies against the parasites, but not all animals seroconverted, highlighting potential limitations in the effectiveness of the chemoprophylaxis approach.
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  • * A study in Rio Grande do Sul, Brazil, revealed that 98% of cattle farms had ticks resistant to multiple acaricides, with 71% of samples resistant to all six tested compounds.
  • * Factors such as the use of injectable acaricides, frequent treatments, and larger herd sizes were linked to increased resistance rates, indicating a serious challenge for effective tick management.
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Background: Brown dog ticks (Rhipicephalus sanguineus sensu lato) are vectors of pathogens adversely affecting the health of dogs in many regions of the world. The three-host life cycle of R. sanguineus s.

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Background: Acaricide resistance in cattle ticks is a significant concern in (sub)tropical regions, particularly Brazil. The Larval Packet Test (LPT) is the standard laboratory bioassay for resistance diagnosis, which requires triplicates of seven acaricidal dilutions plus controls to cover larval mortalities ranging between 0 and 100%. The value of the LPT lies in providing resistance ratios based on the ratio between the LC50 calculated with potentially resistant and susceptible ticks.

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