Successful pregnancy with laparoscopic oocyte retrieval and in-vitro fertilisation in mullerian agenesis.

A 28-year-old woman with mullerian agenesis presented with primary infertility and was considered for laparoscopic oocyte retrieval and in-vitro fertilisation. Her 27-year-old younger sister served as a gestational carrier. The patient underwent ovarian stimulation and 11 mature oocytes were retrieved by laparoscopy.

Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates.

Human day 3 embryos were vitrified in modified ethylene glycol-based protocol. The present study reports a post-thaw survival rate of 95.3%, which was significantly higher than that of the conventional slow-freezing protocol (60.

Effect of prostaglandin F2 alpha on libido, seminal quantity and quality of buffalo bulls.

Four mature Murrah buffalo bulls on a regular semen collection schedule of twice a day, two days per week, were injected with 30 mg prostaglandin F2 alpha THAM salt(PGF) intramuscularly 30 minutes prior to each alternate first ejaculation for six weeks. Effects of PGF on time to initial false mount on the decoy, time to ejaculation after two false mounts (reaction time), seminal volume, sperm concentration, total sperm output, motility of fresh and thawed semen and the semen doses obtained per collection were evaluated. Treatment caused significant (P < 0.