Correlation between antibodies to gonadotropin-releasing hormone and reproductive disorders in humans.

Antibodies (IgG) to gonadotropin-releasing hormone were measured by ELISA in blood serum of women with miscarriage and men with infertility. Spontaneous production of antibodies to gonadotropin-releasing hormone was observed during miscarriage and male infertility: the mean level of antibodies in these groups was higher than in healthy women and men (p<0.0001).

Free radical generation in ejaculate samples from infertile patients.

Generation of free radicals in ejaculate samples from infertile patients was evaluated using chemiluminescent technique. The presence of antisperm antibodies in samples increased the possibility of damages to spermatozoon plasma membranes due to excessive generation of free radicals.

[The clinical use of andriol in the hormonal therapy of male sterility].

The paper presents the experience in administration of testosterone andekanoate (andriol) in males suffering from infertility due to idiopathic oligozoospermia. Andriol produced a positive effect on ejaculate. As a result, 25% of the wives got pregnant.

[Diagnosis and treatment in infertile marriage].

The paper gives data on the frequency and pattern of infertile marriage by referrals to the clinic of infertility, Center of Obstetrics, Gynecology, and Perinatology, Russian Academy of Medical Sciences. Tubuloperitoneal disorders are the leading and challenging abnormality in aphoria. Endometriosis and endocrine types of infertility occupy the second place in their incidence.

Detection of antisperm antibodies on the surface of living spermatozoa using flow cytometry: preliminary study.

Objective: To assess the use of flow cytometry (FCM) analysis of living spermatozoa subjected to indirect immunofluorescence staining with the mouse anti-human immunoglobulin (Ig)G monoclonal antibodies (FCM test) as an objective method for the detection of antisperm antibodies in semen and serum.

Design: The comparative studies were conducted with the direct and indirect mixed antiglobulin reaction (MAR) and FCM test performed by FCM analyzer FACSCAN (Becton Dickinson Immunocytometry Systems, Mountain View, CA).

Setting: Clinical infertile service.